Hi,
Do any of you have any text to contribute towards the WP9 update?
Horizon, can you send me something describing your preparation for the human iPS cell targeting constructs?
Saverio, any progress?
Thanks
Brian
Hi All,
We need to source some human pluripotent cell lines that we can use for manipulation and analysis, given the withdrawal of Cellartis from 4DCellFate. The permits we have from both the UK and European powers that be regarding human ES cell use specify that we are only allowed to use lines that would have been provided by Cellartis. In order to use any other hES line, we'll have to modify this paperwork and await permission.
Therefore I suggest we get started using a human iPS line. To be honest I'm not sure how much benefit there is in using hES vs hiPS for these studies, given the general lack of clarity about exactly what a human "Pluripotent" cell is anyway.
Austin Smith has confirmed that he is willing to provide both an iPS and/or a hES line for our work. So, assuming no one has any objections, I'll get the iPS line and Horizon can get started targeting this line now. I can also apply to use Austin's hES line, but whether we actually use it or not depends upon whether anyone has strong reasons for doing things in hES cells rather that iPS cells.
I'd be happy to hear your thoughts on this.
Best wishes,
Brian
Brian D. Hendrich, PhD
Wellcome Trust Senior Research Fellow
Wellcome Trust-MRC Stem Cell Institute
Department of Biochemistry
University of Cambridge
Tennis Court Road
Cambridge
CB2 1QR
United Kingdom
Office: +44 (0)1223 760205
Lab: +44 (0)1223 760212
Fax: +44 (0)1223 760241
Brian.Hendrich(a)cscr.cam.ac.uk
www.stemcells.cam.ac.uk
HI All,
Time to get this party started, as they say.
One of the first things we could do (while some of us are trying to recruit staff) would be to see if we can get Michiel's human BACs into human ES cells, which would then be used to purify complexes for Mass Spec.
Michiel, you said you needed to check that you can give these to Cellartis for this purpose. Have you managed to do that? If so, can you please arrange to send some BAC DNA to Cellartis?
Lindsay, to whom should they be sent?
Thanks.
Looking forward to some productive collaborations.
Best wishes,
Brian
