Dear all,
This is a reminder to upload the quantification details for the five common samples (see old message below). So far it is only CRG/PCB and UNIGE who have provided information.
I kindly ask you to have a look at the data you have and up-load it or e-mail it to me directly. It is not much data we need.. e.g. our (UU) data looks like this:
Sample Konc.Qubit ng/µl Konc. Bioanalyzer ng/µl HG00117 1280 1460 HG00355 1050 1290 NA06986 1490 not analyzed NA19095 1280 1440 NA20527 1900 not analyzed

Please also mention what method you used for the quantification (Qubit, picogreen, nanodrop etc)
Thanks in advance
Mathias

Hi all,
As part of the inter-lab comparison of the results I was thinking on also comparing the QC performed on the RNA delivered from Geneva.
I suppose that most of the sequencing centres do some kind QC before proceeding with fragmentation and library prep (e.g. we do qPCR and Bioanalyzer).
If you have e.g. the concentrations and RIN-values that you determined for the five common samples (HG00117, HG00355, NA06986, NA19095, NA20527), could you please up-load this as well to the ftp-site?
Remember to specify what method/instrument you used to get the values so that we can do a "fair" comparison.

I suggest you put it in the /upload/geuvadis/wp4_rnaseq/main_project/sample/input_qc folder I just made for this purpose.

Thanks and have a nice weekend,
Mathias

-- 
_____________________________________________
Mathias Brännvall, PhD
Project coordinator
Dept. of Medical Sciences, sect. Molecular Medicine, 
Uppsala University
phone:  +46-(0)18-6114934
mobile: +46-(0)70-1679446

e-mail: Mathias.Brannvall@medsci.uu.se