Dear all,
Here are minutes of the Geuvadis analysis group TC on January 12.
The next call has to be pushed forward by one week to February 2
at 2pm. We'll continue with the normal schedule after that,
so the one after that will be February 9.
best regards,
Tuuli
Action items:
- Tuuli:
Set up a call for next week to discuss imputation details with
Natalja, Micha, Tim.
Continue masked mapping tests
- Esther, Peter, Natalja:
Send an outline of you miRNA analysis pipeline to the analysis
group mailing list
- Everyone:
Think and discuss in your lab about the analyses that you'd want
to do (lead and/or participate) with this dataset, by the next call
- Micha:
Continue mapping tests
- Attending: Tuuli, Micha, Esther, Ivo, Tim, Thomas x2,
Mathias, Peter, Olof, Matthias, Natalja
- Sequencing: Most of the labs will be able to meet the
initial deadline of Feb 15 despite the delays in kit delivery, which
is great. We agreed to keep the very last deadline of March 15; all
the samples and reruns should be done and data submitted by then.
- Genotypes: We need to impute about 40 samples that are not
in 1000g Phase 1 (they have Omni 2.5M haplotypes). Natalia/EBI could
probably do this, and Tuuli, Micha and Tim & co. want to be
involved/informed too. This should be done within a month.
- miRNA analysis pipeline: The labs who have experience of
miRNA analysis will send around details of how they map and quantify
miRNAs. We'll discuss on the next call how we should analyze them.
- A new collaboration: Mark McMarthy from Oxford is a
long-term collaborator of Manolis. They've developed a new kind of a
burden test to look at rare variant effects, and expressed interest
in doing some analysis with the Geuvadis data. People were positive
about this, and Mark's postdoc Manuel Rivas will present their plans
in a future TC.
- Analysis plans: Tuuli would like to coordinate the future
analyses so that the essentials get done and there's no unnecessary
overlaps. For this we also need to assign people who will
lead/participate/be informed for each set of analysis. In the next
call we'll start putting together a list of what will be done and by
whom.
- Analysis group face-to-face meeting: It would be good to
get together to discuss in real life once the data is ready and gone
through basic pipelines - maybe around April.
- mRNA mapping: The GEM paper will be submitted soon, so we
might want to use Micha's pipeline for mapping, quantification and
normalization as the basic approach. Micha presented some slides
about an approach that they're developing for SNP calling from
RNAseq data, based on excessive mapping. Tuuli presented results
suggesting that reference allele mapping bias affects exon
quantifications. We should correct for it e.g. by mapping to a
masked reference.
--
Tuuli Lappalainen, PhD
Department of Genetic Medicine and Development
University of Geneva Medical School
CMU / Rue Michel-Servet 1
1211 Geneva 4
Switzerland
Tel. +41-(0)22-3795550
tuuli.lappalainen@unige.ch