Dear all

We are about to sequence the RNA-seq samples. We spike in phiX at 1% for QC reasons. Do people also still reserve one full lane for phiX to improve base calling? What have people done for their GEUVADIS RNA-seq samples?
Best wishes

Peter

Dr. Peter A.C. 't Hoen

Center for Human and Clinical Genetics

Leiden University Medical Center

Postal zone S4-P

PO Box 9600

2300 RC Leiden

The Netherlands

phone: +31-71-5269421

fax: +31-71-5268285

e-mail: p.a.c.hoen@lumc.nl