Hello,
So, I'd like to ask all the labs that have miRNA data to upload 3
fastq files to the ftp site.
I just uploaded our files, and I'll be expecting files from
Barcelona (Marta/Esther/Sergi), Berlin (Marc), Kiel (Matthias). Does
Munich have miRNA data? Please do this asap.
Detailed instructions:
- upload to
ftp-private.ebi.ac.uk/upload/geuvadis/wp4_rnaseq/main_project/working/miRNA_QC
. User geuvadis, password k2vP3kjJ .
- to make the file processing easy, please name the fastq files
according to the naming instructions:
SAMPLE_ID.SeqLabNumber.MI_YYMMDD_Lane_1.fastq.gz . The lab numbers
are: Geneva=1, Barcelona=2, Berlin=3, Kiel=4, Munich=5. E.g. one of
our files is HG00096.1.MI_120104_4_1.fastq.gz
- choose 3 random files, or pick ones that have a little bit
different numbers of total reads
- upload also a readme file with at least your name and email and
the read length
- use raw fastq files without any adaptor trimming, or if you want,
you can trim all the reads to 36 bp
thanks,
Tuuli
--
Tuuli Lappalainen, PhD
Department of Genetic Medicine and Development
University of Geneva Medical School
CMU / Rue Michel-Servet 1
1211 Geneva 4
Switzerland
Tel. +41-(0)22-3795550
tuuli.lappalainen@unige.ch