Dear all
As I said I will not be able to attend the calls at this day and
time. Jean Monlong from my group will bea ttending. He actually
attended today as well
Roderic
Dear all,
Here are minutes of the Geuvadis analysis group TC on January 12.
The next call has to be pushed forward by one week to February
2 at 2pm. We'll continue with the normal schedule after
that, so the one after that will be February 9.
best regards,
Tuuli
Action items:
- Tuuli:
Set up a call for next week to discuss imputation details with
Natalja, Micha, Tim.
Continue masked mapping tests
- Esther, Peter, Natalja:
Send an outline of you miRNA analysis pipeline to the analysis
group mailing list
- Everyone:
Think and discuss in your lab about the analyses that you'd
want to do (lead and/or participate) with this dataset, by the
next call
- Micha:
Continue mapping tests
- Attending: Tuuli, Micha, Esther, Ivo, Tim, Thomas x2,
Mathias, Peter, Olof, Matthias, Natalja
- Sequencing: Most of the labs will be able to meet the
initial deadline of Feb 15 despite the delays in kit delivery,
which is great. We agreed to keep the very last deadline of March
15; all the samples and reruns should be done and data submitted
by then.
- Genotypes: We need to impute about 40 samples that are
not in 1000g Phase 1 (they have Omni 2.5M haplotypes). Natalia/EBI
could probably do this, and Tuuli, Micha and Tim & co. want to
be involved/informed too. This should be done within a month.
- miRNA analysis pipeline: The labs who have experience of
miRNA analysis will send around details of how they map and
quantify miRNAs. We'll discuss on the next call how we should
analyze them.
- A new collaboration: Mark McMarthy from Oxford is a
long-term collaborator of Manolis. They've developed a new kind of
a burden test to look at rare variant effects, and expressed
interest in doing some analysis with the Geuvadis data. People
were positive about this, and Mark's postdoc Manuel Rivas will
present their plans in a future TC.
- Analysis plans: Tuuli would like to coordinate the
future analyses so that the essentials get done and there's no
unnecessary overlaps. For this we also need to assign people who
will lead/participate/be informed for each set of analysis. In the
next call we'll start putting together a list of what will be done
and by whom.
- Analysis group face-to-face meeting: It would be good to
get together to discuss in real life once the data is ready and
gone through basic pipelines - maybe around April.
- mRNA mapping: The GEM paper will be submitted soon, so
we might want to use Micha's pipeline for mapping, quantification
and normalization as the basic approach. Micha presented some
slides about an approach that they're developing for SNP calling
from RNAseq data, based on excessive mapping. Tuuli presented
results suggesting that reference allele mapping bias affects exon
quantifications. We should correct for it e.g. by mapping to a
masked reference.
--
Tuuli Lappalainen, PhD
Department of Genetic Medicine and Development
University of Geneva Medical School
CMU / Rue Michel-Servet 1
1211 Geneva 4
Switzerland
Tel. +41-(0)22-3795550
tuuli.lappalainen@unige.ch
--
(1) Centre de Regulacio Genomica
(2) Universitat Pompeu Fabra
Dr. Aiguader, 88 +34 93 3160110 ph
E-08003 Barcelona +34 93 3969983 fax
email: roderic.guigo@crg.cat http://big.crg.cat
RECOMB 2012 in Barcelona, http://recomb2012.crg.cat/
10 PhD fellowships, http://www.crg.eu/lacaixa_fellowships
3 fellowships for interdisciplinary post-doctoral studies
http://www.crg.es/interpod