Hello,
Here are miRNA QC results of the replicate samples from Matthias.
best regards,
Tuuli
Tuuli Lappalainen, PhD
Department of Genetic Medicine and Development
University of Geneva Medical School
CMU / Rue Michel-Servet 1
1211 Geneva 4
Switzerland
Tel. +41-(0)22-3795550
tuuli.lappalainen@unige.ch
-------- Original Message --------
Hello Tuuli,
could you please try to forward the attached pdf file to the others.
I tried it in three different ways (as excel sheet, zip file and
pdf) but always get an error.
Thanks,
Matthias
-------- Original Message --------
Dear all,
here is the quality analysis we have done on the common miRNA samples.
There's one sample which shows a weird read-length for the genome
mapping (NA20527, page 11-12). However, in the miRNA mapping it's not
that much different anymore, but it misses some more miRNAs (page 6).
And there are a few samples which show almost equal amounts of short
reads (14 bp) compared to the number of 22 bp reads.
Everything else looks quite similar.
One other thing I noticed is, that the sample NA19095 seems to contain a
(very little) bit more poly-A tagged RNAs (page 2, upper graph). This is
probably due to the extraction, as all institutes have roughly the same
amount of polyA-trimmed reads for the same samples.
For mapping only reads were used, which had the adapter successfully
removed.
best wishes,
Matthias
--
Matthias Barann
Institute of Clinical Molecular Biology
Christian Albrechts University Kiel
Schittenhelmstr. 12
D-24105 Kiel, Germany
m.barann@ikmb.uni-kiel.de
+49 - (0)431 - 597 8681 (office)