[Geuvadis_rnaseq] Geuvadis mRNAseq kit - further specification

Dear all, I forgot to specify before that we should all use the high-throughput protocol in the library preparation - thanks Peter for pointing this out. No one has had any objections about the kit specified below, so we'll use that in the Geuvadis library preps. best regards, Tuuli Tuuli Lappalainen, PhD Department of Genetic Medicine and Development University of Geneva Medical School CMU / Rue Michel-Servet 1 1211 Geneva 4 Switzerland Tel. +41-(0)22-3795550 tuuli.lappalainen(a)unige.ch -------- Original Message -------- Subject: Geuvadis mRNAseq kit Date: Tue, 27 Sep 2011 10:29:00 +0200 From: Tuuli Lappalainen <Tuuli.Lappalainen(a)unige.ch> To: geuvadis_rnaseq(a)lists.crg.es <geuvadis_rnaseq(a)lists.crg.es> Dear all, For the mRNA sequencing of the Geuvadis samples, we suggest using the latest Illumina sample prep kit for HiSeq with 24 indexes: TruSeq RNA Sample Prep Kit V2 Set A and Set B (RS-122-2001 and RS-122-2002) As we agreed on the call, you should let me know this week if you don't agree with using this kit - otherwise we'll go forward with this, and everyone should to order the kits as necessary to prepare for receiving the RNAs in the end of October or early November. Please see the attachment for full information from Illumina for ordering - as you know, it is absolutely critical that we all follow the same protocol. best regards, Tuuli -- Tuuli Lappalainen, PhD Department of Genetic Medicine and Development University of Geneva Medical School CMU / Rue Michel-Servet 1 1211 Geneva 4 Switzerland Tel. +41-(0)22-3795550 tuuli.lappalainen(a)unige.ch