unfortunately for you, I am on holidays next week. I
was in the call yesterday and I think it is critical
that we nail this experiment down absolutely right,
otherwise we will certainly fail. This experiment is
too expensive for us to fail !!!
On 19/04/2011 10:42, Thomas Giger wrote:
Dear All,
As we decided yesterday, the people involved
in the RNAseq related part of the experiment
should have a conference call to discuss open
questions.
I have written down the following points that
should be discussed:
1. As of now we made plans that the different
labs analyze samples from one particular
population (as listed in the workflow document
that I've sent around on April 7).
It would be a better experimental design if
the samples were randomly distributed among the
different labs. Since there is still time to
react - do we want to switch the strategy?
2. How long are the library prep / sequencer
queue up times in the different labs (once you
receive the RNA samples - how long do you think
will it take you to analyze them?).
If some labs expect to have much larger
delays, we could make arrangements that those
labs get samples earlier than other labs.
This would also mean that we would all use
the protocol that is used at the time point when
the first lab starts to analyze samples.
3. Which other samples (other than the 500
1KGP European samples) should be analyzed by
RNAseq?
4. Specifications for the small RNA protocol.
If you know already now, that you would like
to have additional things discussed - please let
me know and I can make sure that we don't forget
about.
We would like to schedule the call for either
next Tuesday (April 26) or Wednesday (April
27).
cheers,
Thomas
------------------------------------------------------------------------
Thomas Giger, Ph.D.
Department of Genetic Medicine and
Development
University of Geneva Medical School
1 Rue Michel-Servet
Geneva 1211
Switzerland
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