That's odd!
In my view there is no problem and I don't see a difference

Cheers,
Manolis

------------------------------------------
Emmanouil (Manolis) Dermitzakis, PhD
Louis-Jeantet Professor
Department of Genetic Medicine and Development
University of Geneva Medical School
1 Rue Michel-Servet (CMU office 9088)
Geneva 1211
Switzerland

Email: emmanouil.dermitzakis@unige.ch
Tel: +41 (0) 22 379 5483
Fax: +41 (0) 22 379 5706
website: http://funpopgen.unige.ch/

Admin assistant: Ancilla Stefani,
Tel: +41 22 379 5551
e-mail: Ancilla.Stefani@unige.ch
------------------------------------------




P.A.C._t_Hoen@lumc.nl wrote:

Dear Tuuli

Our sequence facility only sequences 2x100 and not 2x75. Is it allowed

to sequence 100bp and throw away the last 25 nucleotides?

Best

Peter

------------------------------------------------------------------------

*From:* geuvadis_rnaseq-bounces@lists.crg.es

[mailto:geuvadis_rnaseq-bounces@lists.crg.es] *On Behalf Of *Tuuli

Lappalainen

*Sent:* Thursday, November 03, 2011 7:26 PM

*To:* geuvadis_rnaseq@lists.crg.es

*Subject:* [Geuvadis_rnaseq] Geuvadis RNAseq Minutes

Dear all,

Enclosed are the Minutes of this afternoon's TC. Action items

copypasted below.

best regards,

Tuuli

*Action items*

-    *Tuuli* will take care of the RNA sample shipments (7/14

November) and inform the contact persons from each lab when the

samples are shipped

-    *Esther* from Barcelona will email Tuuli further specifications

of the miRNAseq protocol, including the minimum coverage per sample

and how exactly that is defined

-    *Tuuli* will send the final version of the sequencing guidelines

to all the labs by November 10

-    *Gabrielle* will set up a mailing list for the analysis group

-    After the labs have received the samples, there is one major

action item for *all partners*: sequencing!

--

Tuuli Lappalainen, PhD

Department of Genetic Medicine and Development

University of Geneva Medical School

CMU / Rue Michel-Servet 1

1211 Geneva 4

Switzerland

Tel. +41-(0)22-3795550

tuuli.lappalainen@unige.ch <mailto:tuuli.lappalainen@unige.ch>

------------------------------------------------------------------------

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