Dear all,

This email contains further guidelines for miRNA seq. The partner labs should now move forward with miRNAseq as with mRNAseq, without further waiting for additional instructions.

- the coverage is minimum 3M total production reads (from a good-quality experiment)
- You should follow the protocol of the TruSeq Sm RNA Sample Prep kit with parameters that work in your lab. We are doing the optional ethanol precipitation step after the gel, but we don't require everyone to do this if all works well without it.
- 36bp single-end sequencing, fragment size 145-160 bp
- there was a typo in the guidelines: the Cluster generation kit will of course be TruSeq SR Cluster Kit v3, not PE
- deadline for both miRNAseq and mRNAseq data is February 15

The updated sequencing guidelines document is enclosed.

Don't hesitate to ask if you have any questions, and see you soon in Toulouse.

best regards,
Tuuli

-- 
Tuuli Lappalainen, PhD
Department of Genetic Medicine and Development
University of Geneva Medical School
CMU / Rue Michel-Servet 1
1211 Geneva 4
Switzerland
Tel. +41-(0)22-3795550
tuuli.lappalainen@unige.ch