unfortunately for you, I am on holidays next week. I was in the call
yesterday and I think it is critical that we nail this experiment
down absolutely right, otherwise we will certainly fail. This
experiment is too expensive for us to fail !!!
Dear All,
As we decided yesterday, the people involved in the RNAseq
related part of the experiment should have a conference call to
discuss open questions.
I have written down the following points that should be
discussed:
1. As of now we made plans that the different labs analyze
samples from one particular population (as listed in the
workflow document that I've sent around on April 7).
It would be a better experimental design if the samples were
randomly distributed among the different labs. Since there is
still time to react - do we want to switch the strategy?
2. How long are the library prep / sequencer queue up times
in the different labs (once you receive the RNA samples - how
long do you think will it take you to analyze them?).
If some labs expect to have much larger delays, we could make
arrangements that those labs get samples earlier than other
labs.
This would also mean that we would all use the protocol that
is used at the time point when the first lab starts to analyze
samples.
3. Which other samples (other than the 500 1KGP European
samples) should be analyzed by RNAseq?
4. Specifications for the small RNA protocol.
If you know already now, that you would like to have
additional things discussed - please let me know and I can make
sure that we don't forget about.
We would like to schedule the call for either next Tuesday
(April 26) or Wednesday (April 27).
cheers,
Thomas
------------------------------------------------------------------------
Thomas Giger, Ph.D.
Department of Genetic Medicine and Development
University of Geneva Medical School
1 Rue Michel-Servet
Geneva 1211
Switzerland
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