28 Apr
2013
28 Apr
'13
10:12 p.m.
Dear all
I just submitted the revised version of the QC paper. Attached the most important files.
An important last minute addition, with possible consequences for current sequencing
practice in our laboratories, is the relatively poor representation of exons with >65%
in laboratories CNAG, MPIMG, and HMGU (Suppl Figure 6B). This is likely an effect of
thermocyclers with high ramp speed, which are actually too fast for proper melting of high
GC products. See also Aird et al. Genome Biol 2011.
Thank you very much for all your contributions and let's hope for the best
Best wishes
Peter
28 Apr
28 Apr
10:47 p.m.
Dear Peter,
Excellent work. Thanks for the comment on the representation on exons.
Best regards,
Xavier
El Apr 28, 2013, a les 10:12 PM,
<P.A.C._t_Hoen@lumc.nl<mailto:t_Hoen@lumc.nl>> va escriure:
Dear all
I just submitted the revised version of the QC paper. Attached the most important files.
An important last minute addition, with possible consequences for current sequencing
practice in our laboratories, is the relatively poor representation of exons with >65%
in laboratories CNAG, MPIMG, and HMGU (Suppl Figure 6B). This is likely an effect of
thermocyclers with high ramp speed, which are actually too fast for proper melting of high
GC products. See also Aird et al. Genome Biol 2011.
Thank you very much for all your contributions and let’s hope for the best
Best wishes
Peter
<37715_1_merged_1367178637.pdf><tHoen_et_al_Suppl_Figures.pdf>
29 Apr
29 Apr
9:45 a.m.
Hoi PB. Goed gedaan! Met die >65% bedoel je wsch >65% GC? Gr GJ
Sent from handheld, sorry for typo's
From: Hoen, P.A.C. 't (HG)
Sent: Sunday, April 28, 2013 10:12 PM
To: 'Jonas Carlsson' <jonas.carlsson(a)medsci.uu.se>se>; 'Marc
Friedlaender' <Marc.Friedlaender(a)crg.eu>eu>; 'M.Sammeth'
<micha(a)sammeth.net>et>; Pulyakhina, I. (HG); Laros, J.F.J. (HG); Anvar, S.Y. (HG);
'olof.karlberg(a)medsci.uu.se' <olof.karlberg(a)medsci.uu.se>se>;
'Ann-Christine Syvänen' <ann-christine.syvanen(a)medsci.uu.se>se>; 'Roderic
Guigo' <roderic.guigo(a)crg.cat>at>; 'xavier.estivill(a)crg.eu'
<xavier.estivill(a)crg.eu>eu>; Ommen, G.J.B. van (HG); 'Manolis Dermitzakis'
<Emmanouil.Dermitzakis(a)unige.ch>ch>; 'Tuuli.Lappalainen(a)unige.ch'
<Tuuli.Lappalainen(a)unige.ch>ch>; Mathias Brännvall (mathias.brannvall(a)medsci.uu.se)
<mathias.brannvall(a)medsci.uu.se>se>; Buermans, H.P.J. (HG); Dunnen, J. den
Cc: geuvadis_rnaseq(a)lists.crg.es <geuvadis_rnaseq(a)lists.crg.es>es>; Hoen, P.A.C. 't
(HG)
Subject: GEUVADIS QC paper submitted
Dear all
I just submitted the revised version of the QC paper. Attached the most important files.
An important last minute addition, with possible consequences for current sequencing
practice in our laboratories, is the relatively poor representation of exons with >65%
in laboratories CNAG, MPIMG, and HMGU (Suppl Figure 6B). This is likely an effect of
thermocyclers with high ramp speed, which are actually too fast for proper melting of high
GC products. See also Aird et al. Genome Biol 2011.
Thank you very much for all your contributions and let’s hope for the best
Best wishes
Peter
4161
days inactive
4162
days old
2 comments
3 participants
participants (3)
-
G.J.B.van_Ommen＠lumc.nl -
P.A.C._t_Hoen＠lumc.nl -
Xavier Estivill