Dear all,
This email contains further guidelines for miRNA seq. The partner labs
should now *move forward with miRNAseq* as with mRNAseq, without further
waiting for additional instructions.
- the coverage is minimum 3M *total* production reads (from a
good-quality experiment)
- You should follow the protocol of the TruSeq Sm RNA Sample Prep kit
with parameters that work in your lab. We are doing the optional ethanol
precipitation step after the gel, but we don't require everyone to do
this if all works well without it.
- 36bp single-end sequencing, fragment size 145-160 bp
- there was a typo in the guidelines: the Cluster generation kit will of
course be TruSeq *SR* Cluster Kit v3, not PE
- deadline for both miRNAseq and mRNAseq data is February 15
The updated sequencing guidelines document is enclosed.
**
Don't hesitate to ask if you have any questions, and see you soon in
Toulouse.
best regards,
Tuuli
--
Tuuli Lappalainen, PhD
Department of Genetic Medicine and Development
University of Geneva Medical School
CMU / Rue Michel-Servet 1
1211 Geneva 4
Switzerland
Tel. +41-(0)22-3795550
tuuli.lappalainen(a)unige.ch