Hi all - especially Thasso and Micha,
In order to correct/filter for allelic mapping bias in Geuvadis analysis
as we've discussed before, I've simulated reads over 1000g variants.
The fastq files are available for mapping in
/upload/geuvadis/wp4_rnaseq/main_project/external_data/genetic_variation/allelic_mapping_simulation/fastq
. These should be mapped with GEM and converted to bam similarly to our
real data, except that these are single-end reads. It actually became a
lot less reads than I anticipated because I ended up taking only sites
that are polymorphic in our populations: it's about 300M reads in total,
so I hope that this could be done pretty soon :-) At least, could you
please check already this week that the files are OK so that I can
modify them if needed without further delay.
Further details in the wiki:
http://www.geuvadis.org/group/geuvadis/wikis/-/wiki/Main%20Geuvadis%20Wiki/…
cheers,
Tuuli
--
Tuuli Lappalainen, PhD
Department of Genetic Medicine and Development
University of Geneva Medical School
CMU / Rue Michel-Servet 1
1211 Geneva 4
Switzerland
Tel. +41-(0)22-3795550
tuuli.lappalainen(a)unige.ch