[Geuvadis_rna_analysis] miRNA quality comparison

Dear all, here is our analysis of the miRNA samples of 4 institutes (UNIGE, MPIMG, ICMB, CNAG, see attatched pdf file). This is basically what I did: 1. trim reads to 36 bp (prinseq). 2. remove adapter sequence (cutadapt), split into trimmed reads and untrimmed reads 3. remove polyA tails (cutadapt) 4. map trimmed reads against genome and miRNA reference (the one from the FTP server). 5. count reads per miRNA There are larger differences considering the amount of adapter sequences within the samples and the library diversity. best wishes, Matthias -- Matthias Barann Institute of Clinical Molecular Biology Christian Albrechts University Kiel Schittenhelmstr. 12 D-24105 Kiel, Germany m.barann(a)ikmb.uni-kiel.de +49 - (0)431 - 597 8681 (office)