[Geuvadis_rna_analysis] miRNA sample QC

Hello, I've done basic QC for miRNA samples. Attached is a sample sheet that denotes samples to use in analysis. From the total 492 samples, 480 samples from 452 unique individuals pass QC. We end up having 705 genes quantified in at least 50% of the samples - most of these have low quantifications though. Analysis of sample correlation and clustering shows even a clearer population clustering than mRNA, and very little lab effects. I've updated the QC section of the wiki with miRNA results: list of samples to exclude, basic read count and mapping stats, and early correlation analysis. The data is on the ftp site: - sample sheet: /upload/geuvadis/wp4_rnaseq/main_project/analysis_data/qc - quantifications: /upload/geuvadis/wp4_rnaseq/main_project/analysis_data/mirna/quantification_tables I'm now running PEER normalization for miRNAs, and that data will probably be the best to use for mRNA-miRNA correlations and eQTL analysis. I'll let you know when this is done. cheers, Tuuli -- Tuuli Lappalainen, PhD Department of Genetic Medicine and Development University of Geneva Medical School CMU / Rue Michel-Servet 1 1211 Geneva 4 Switzerland Tel. +41-(0)22-3795550 tuuli.lappalainen(a)unige.ch