6 Feb
2012
6 Feb
'12
2:44 p.m.
Same for us in Uppsala. We use PhiX but not in a lane of its own.
atb
/mathias
On 2012-02-06 13:32, Manolis Dermitzakis wrote:
Hi Peter
We don't use a separate lane for PhiX.
Cheers,
Manolis
------------------------------------------
Emmanouil (Manolis) Dermitzakis, PhD
Louis-Jeantet Professor
Department of Genetic Medicine and Development
University of Geneva Medical School
1 Rue Michel-Servet (CMU office 9088)
Geneva 1211
Switzerland
Email: emmanouil.dermitzakis(a)unige.ch
Tel: +41 (0) 22 379 5483
Fax: +41 (0) 22 379 5706
website: http://funpopgen.unige.ch/
Admin assistant: Ancilla Stefani,
Tel: +41 22 379 5551
e-mail: Ancilla.Stefani(a)unige.ch
------------------------------------------
P.A.C._t_Hoen(a)lumc.nl wrote:
--
_____________________________________________
Mathias Brännvall, PhD
Project coordinator
Dept. of Medical Sciences, sect. Molecular Medicine,
Uppsala University
phone: +46-(0)18-6114934
mobile: +46-(0)70-1679446
e-mail: Mathias.Brannvall(a)medsci.uu.se
Dear all
We are about to sequence the RNA-seq samples. We spike in phiX at 1%
for QC reasons. Do people also still reserve one full lane for phiX to
improve base calling? What have people done for their GEUVADIS RNA-seq
samples?
Best wishes
Peter
Dr. Peter A.C. 't Hoen
Center for Human and Clinical Genetics
Leiden University Medical Center
Postal zone S4-P
PO Box 9600
2300 RC Leiden
The Netherlands
phone: +31-71-5269421
fax: +31-71-5268285
e-mail: p.a.c.hoen(a)lumc.nl
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