Hello,
in BCN we never sequence on the HS without equilibrate genome lane used
as a control lane, as Illumina recommends. We never use PhiX in entire
lane for this purpose since it is not mandatory from Illumina, this used
to be few years back for the GA. All the lanes are PhiX spiked in.
Unfortunately, for the Geuvadis smallRNA due to the index distribution
the run is without a CTRL lane, that I find as quite an nuance. Though
the run is of excellent quality we are trying to use the previous matrix
to improve the base calling.
For sure the base calling without equilibrate genome lane used as a
control lane is possible, however, it is lower quality.
Best,
Marta
On 06/02/2012 14:44, Mathias Brännvall wrote:
Same for us in Uppsala. We use PhiX but not in a lane
of its own.
atb
/mathias
On 2012-02-06 13:32, Manolis Dermitzakis wrote:
> Hi Peter
> We don't use a separate lane for PhiX.
> Cheers,
> Manolis
>
> ------------------------------------------
> Emmanouil (Manolis) Dermitzakis, PhD
> Louis-Jeantet Professor
> Department of Genetic Medicine and Development
> University of Geneva Medical School
> 1 Rue Michel-Servet (CMU office 9088)
> Geneva 1211
> Switzerland
>
> Email: emmanouil.dermitzakis(a)unige.ch
> Tel: +41 (0) 22 379 5483
> Fax: +41 (0) 22 379 5706
> website:
http://funpopgen.unige.ch/
>
> Admin assistant: Ancilla Stefani,
> Tel: +41 22 379 5551
> e-mail: Ancilla.Stefani(a)unige.ch
> ------------------------------------------
>
>
>
>
> P.A.C._t_Hoen(a)lumc.nl wrote:
>> Dear all
>>
>> We are about to sequence the RNA-seq samples. We spike in phiX at 1%
>> for QC reasons. Do people also still reserve one full lane for phiX to
>> improve base calling? What have people done for their GEUVADIS RNA-seq
>> samples?
>> Best wishes
>>
>> Peter
>>
>>
>>
>>
>>
>> Dr. Peter A.C. 't Hoen
>>
>> Center for Human and Clinical Genetics
>>
>> Leiden University Medical Center
>>
>> Postal zone S4-P
>>
>> PO Box 9600
>>
>> 2300 RC Leiden
>>
>> The Netherlands
>>
>>
>>
>> phone: +31-71-5269421
>>
>> fax: +31-71-5268285
>>
>> e-mail: p.a.c.hoen(a)lumc.nl
>>
>>
>>
>> ------------------------------------------------------------------------
>>
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--
Marta Gut, PhD
Head of Sequencing dept.
Centro nacional de análisis genómico (CNAG)
Parc Científic de Barcelona , Torre I
c/Baldiri i Reixac, 6;
Barcelona 08028
tel +34 93 4020568
fax +34 93 4037279