Hello,
A couple of data and analysis updates:
- QC: I've reran the plots of sample correlations and clustering using
the correlation matrices provided by Peter. The total dataset shows
clustering by lab rather than populations (probably because systematical
differences between these populations are not that big), but the
replicate samples look very good with lab effects being less than
biological variation. See
http://sanabre.net/geuvadis/index.php/MRNA#Sample_correlation_and_clusterin…
- Splice junctions for Manny: I've normalized the junctions by total
gene count, to able able to extract splice changes from variation in
overall expression level. The files of these with subsequent
transformation to standard normal are in
/upload/geuvadis/wp4_rnaseq/main_project/analysis_data/quantification/splice_junction-intron
, file names with GeneN.
have a nice weekend,
Tuuli
--
Tuuli Lappalainen, PhD
Department of Genetic Medicine and Development
University of Geneva Medical School
CMU / Rue Michel-Servet 1
1211 Geneva 4
Switzerland
Tel. +41-(0)22-3795550
tuuli.lappalainen(a)unige.ch