[Geuvadis_rna_analysis] analysis_data/quantification/transcript

Dear all, we just replaced the transcript quantification files in analysis_data/quantification/transcript because we found that a script which counted the number of mapped reads for the RPKM normalization got confused by the whitespace characters inserted by the new casava/Illumina pipeline into the read IDs. Therefore, the RPKM values in the former files were off by ~ factor 2, the exact ratio old/new varies expectedly few % around the median 1.92 (plot attached). Hopefully this lapsus did not affect anybody's work--and please be careful not to run into the same issue when emplyoing programs that split columns by whitespaces on files with these read IDs; that was really a great idea of Illumina. Best, micha -- Dr. Michael Sammeth, GL Functional Bioinformatics Phone: +34-934-020-580, http://www.cnag.eu Centre Nacional d'Anàlisi Genòmica (CNAG), PCB Baldiri Reixac 4-6, Room 02A8, 08028 Barcelona