Hello,
Here are some preliminary smallRNA QC stats from the 5 sandbox samples
sequenced in Geneva:
1.9-3.5M total reads
Adaptor trimmed with cutadapt software. See the attached plot for read
length distribution
Mapping with bwa to 3 different references. No MAPQ filter
95-97% map to the genome
11.1-16.7% map to miRNA hairpin sequences
6.6-8.5% map to mature miRNA sequences
best regards,
Tuuli
Tuuli Lappalainen, PhD
Department of Genetic Medicine and Development
University of Geneva Medical School
CMU / Rue Michel-Servet 1
1211 Geneva 4
Switzerland
Tel. +41-(0)22-3795550
tuuli.lappalainen(a)unige.ch
On 2/8/12 6:23 PM, Tuuli Lappalainen wrote:
Hello all,
We have the Geuvadis analysis group TC on Thursday (Feb 8) at 2pm.
On the agenda:
- updates on sequencing if needed
- analysis group face-to-face meeting
*Please fill the Doodle!
http://www.doodle.com/24a4i3n8bkynhpwx
*- small RNA QC
- Manuel Rivas will present some slides on LOF analysis plans
Here are the call details:
Spain
902 125 136
Other countries
+34 91 495 18 99
Participants' Pin-code:
764989*
best regards,
Tuuli
--
Tuuli Lappalainen, PhD
Department of Genetic Medicine and Development
University of Geneva Medical School
CMU / Rue Michel-Servet 1
1211 Geneva 4
Switzerland
Tel. +41-(0)22-3795550
tuuli.lappalainen(a)unige.ch
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