[Geuvadis_rna_analysis] Fwd: Fwd: Re: Geuvadis RNAseq analysis group TC - slides

Hello, Here are miRNA QC results of the replicate samples from Matthias. best regards, Tuuli Tuuli Lappalainen, PhD Department of Genetic Medicine and Development University of Geneva Medical School CMU / Rue Michel-Servet 1 1211 Geneva 4 Switzerland Tel. +41-(0)22-3795550 tuuli.lappalainen(a)unige.ch -------- Original Message -------- Subject: Fwd: Re: [Geuvadis_rna_analysis] Geuvadis RNAseq analysis group TC - slides Date: Thu, 23 Feb 2012 16:28:23 +0100 From: Matthias Barann <m.barann(a)ikmb.uni-kiel.de> To: Tuuli Lappalainen <Tuuli.Lappalainen(a)unige.ch> Hello Tuuli, could you please try to forward the attached pdf file to the others. I tried it in three different ways (as excel sheet, zip file and pdf) but always get an error. Thanks, Matthias -------- Original Message -------- Subject: Re: [Geuvadis_rna_analysis] Geuvadis RNAseq analysis group TC - slides Date: Thu, 23 Feb 2012 16:26:39 +0100 From: Matthias Barann <m.barann(a)ikmb.uni-kiel.de> To: geuvadis_rna_analysis(a)lists.crg.es Dear all, here is the quality analysis we have done on the common miRNA samples. There's one sample which shows a weird read-length for the genome mapping (NA20527, page 11-12). However, in the miRNA mapping it's not that much different anymore, but it misses some more miRNAs (page 6). And there are a few samples which show almost equal amounts of short reads (14 bp) compared to the number of 22 bp reads. Everything else looks quite similar. One other thing I noticed is, that the sample NA19095 seems to contain a (very little) bit more poly-A tagged RNAs (page 2, upper graph). This is probably due to the extraction, as all institutes have roughly the same amount of polyA-trimmed reads for the same samples. For mapping only reads were used, which had the adapter successfully removed. best wishes, Matthias -- Matthias Barann Institute of Clinical Molecular Biology Christian Albrechts University Kiel Schittenhelmstr. 12 D-24105 Kiel, Germany m.barann(a)ikmb.uni-kiel.de +49 - (0)431 - 597 8681 (office)