Same for us in Uppsala. We use PhiX but not in a lane of its own. atb /mathias On 2012-02-06 13:32, Manolis Dermitzakis wrote: > Hi Peter > We don't use a separate lane for PhiX. > Cheers, > Manolis > > ------------------------------------------ > Emmanouil (Manolis) Dermitzakis, PhD > Louis-Jeantet Professor > Department of Genetic Medicine and Development > University of Geneva Medical School > 1 Rue Michel-Servet (CMU office 9088) > Geneva 1211 > Switzerland > > Email: emmanouil.dermitzakis(a)unige.ch > Tel: +41 (0) 22 379 5483 > Fax: +41 (0) 22 379 5706 > website: http://funpopgen.unige.ch/ > > Admin assistant: Ancilla Stefani, > Tel: +41 22 379 5551 > e-mail: Ancilla.Stefani(a)unige.ch > ------------------------------------------ > > > > > P.A.C._t_Hoen(a)lumc.nl wrote: >> Dear all >> >> We are about to sequence the RNA-seq samples. We spike in phiX at 1% >> for QC reasons. Do people also still reserve one full lane for phiX to >> improve base calling? What have people done for their GEUVADIS RNA-seq >> samples? >> Best wishes >> >> Peter >> >> >> >> >> >> Dr. Peter A.C. 't Hoen >> >> Center for Human and Clinical Genetics >> >> Leiden University Medical Center >> >> Postal zone S4-P >> >> PO Box 9600 >> >> 2300 RC Leiden >> >> The Netherlands >> >> >> >> phone: +31-71-5269421 >> >> fax: +31-71-5268285 >> >> e-mail: p.a.c.hoen(a)lumc.nl >> >> >> >> ------------------------------------------------------------------------ >> >> _______________________________________________ >> Geuvadis_rna_analysis mailing list >> Geuvadis_rna_analysis(a)lists.crg.es >> http://davinci.crg.es/mailman/listinfo/geuvadis_rna_analysis >> > _______________________________________________ > Geuvadis_rna_analysis mailing list > Geuvadis_rna_analysis(a)lists.crg.es > http://davinci.crg.es/mailman/listinfo/geuvadis_rna_analysis