Hello, Here are miRNA QC results of the replicate samples from Matthias. best regards, Tuuli Tuuli Lappalainen, PhD Department of Genetic Medicine and Development University of Geneva Medical School CMU / Rue Michel-Servet 1 1211 Geneva 4 Switzerland Tel. +41-(0)22-3795550 tuuli.lappalainen(a)unige.ch -------- Original Message -------- Subject: Fwd: Re: [Geuvadis_rna_analysis] Geuvadis RNAseq analysis group TC - slides Date: Thu, 23 Feb 2012 16:28:23 +0100 From: Matthias Barann <m.barann(a)ikmb.uni-kiel.de> To: Tuuli Lappalainen <Tuuli.Lappalainen(a)unige.ch> Hello Tuuli, could you please try to forward the attached pdf file to the others. I tried it in three different ways (as excel sheet, zip file and pdf) but always get an error. Thanks, Matthias -------- Original Message -------- Subject: Re: [Geuvadis_rna_analysis] Geuvadis RNAseq analysis group TC - slides Date: Thu, 23 Feb 2012 16:26:39 +0100 From: Matthias Barann <m.barann(a)ikmb.uni-kiel.de> To: geuvadis_rna_analysis(a)lists.crg.es Dear all, here is the quality analysis we have done on the common miRNA samples. There's one sample which shows a weird read-length for the genome mapping (NA20527, page 11-12). However, in the miRNA mapping it's not that much different anymore, but it misses some more miRNAs (page 6). And there are a few samples which show almost equal amounts of short reads (14 bp) compared to the number of 22 bp reads. Everything else looks quite similar. One other thing I noticed is, that the sample NA19095 seems to contain a (very little) bit more poly-A tagged RNAs (page 2, upper graph). This is probably due to the extraction, as all institutes have roughly the same amount of polyA-trimmed reads for the same samples. For mapping only reads were used, which had the adapter successfully removed. best wishes, Matthias -- Matthias Barann Institute of Clinical Molecular Biology Christian Albrechts University Kiel Schittenhelmstr. 12 D-24105 Kiel, Germany m.barann(a)ikmb.uni-kiel.de +49 - (0)431 - 597 8681 (office)